Ellagic acid pdf

Antioxidant Holm—Sidak Activitymethod. Antioxidant activity 2. It and methanolic was observed extracts. Previous work [15] showed that the The scavenging activity of the pure methanolic extract fruithas was The high and methanol—water extract may be due to the high amounts of caffeic acid present in these extracts. The antioxidant potential of other reported phenolic contribute potentially.

Radical Figure scavenging 4. Values melon seeds. Valuesare are thethe mean ofmean triplicate readings. Materials and Methods 3. Materials Fresh melon was collected from the market, and its seeds were removed.

Sample Collection and Preparation The seeds were removed from the fruits. The seeds were washed with distilled water and were completely dried. The dried seeds were crushed, grinded and converted into finely divided powder. The samples for analysis were prepared from the powdered materials. Briefly, 1 g of powdered sample was mixed with 20 mL of methanol—water mixture. Then, the samples were centrifuged at rpm for 10 min.

The results of TPC for both methods of analyses were compared. The elution was achieved at 25 min due to the elution of all compounds present. The DAD was set to nm for analysis of phenolic compounds. The spectra were recorded from to nm. The identification and quantification of phenolic compound was carried out using available standards, retention times, and their UV spectra. In cases where an authentic standard was not available, compounds were identified by comparing UV spectra with reported literature and quantified using the available relevant standard phenolic compounds based on the similar chromatographic response as given earlier [10].

The reagent was freshly prepared in the lab. Briefly, a sample 0. Absorbance of the resultant mixture was recorded at nm. A Gallic acid standard calibration curve was prepared in the concentration range of 5— mg. DPPH solution of 0. DPPH solution of 1. The mixture was kept in the dark for 30 min and the absorbance was measured at nm using Pharmaspec spectrophotometer Shimadzu, Tokyo, Japan.

Data Analyses All samples were measured in triplicate. Conclusions In conclusion, different extracts of honeydew melon seeds were analyzed for phenolic composition, total phenolic contents and antioxidant activity. The HPLC-DAD results revealed the identification of five phenolic compounds in water extracts, namely gallic acid and its derivative, hydroxybenzoic acid and catechin derivatives, and caffeic acid.

The methanol—water extract has revealed nine phenolic compounds, which include caffeic acids, two vanillic acid derivatives, ellagitanins, Quercetinrutinoside, derivatives of syringic acid and ellagic acid. The amounts of gallic acid, caffeic acid, and catechin were higher among all phenolic compounds. Total phenolic compounds and radical scavenging activity were higher in water extract and methanol—water extract than its corresponding methanol extracts.

These results concluded that melon seeds are a good source of natural antioxidants and may serve as food ingredients and fortification for maintaining shelf life. Conflicts of Interest: The author declares no conflict of interest.

References 1. Eitenmiller, R. Nutrient composition of cantaloupe and honeydew melons. Food Sci. Laur, L. Provitamin A and vitamin C contents in selected California-grown cantaloupe and honeydew melons and imported melons. Food Comps. Lester, G. Antioxidant, sugar, mineral, and phytonutrient concentrations across edible fruit tissues of orange-fleshed honeydew melon Cucumis melo L. Food Chem. Shelf life of minimally processed honeydew, kiwifruit, papaya, pineapple and cantaloupe.

Perry, P. Analysis of honeydew melon Cucumis melo var. Kolayli, S. Comparative study of chemical and biochemical properties of different melon cultivars: Standard, hybrid, and grafted melons. Yanty, N. Physicochemical properties of cucumis melo var. Food Lipids , 15, 42— Petkova, Z. Proximate composition of seeds and seed oils from melon cucumis melo L. Cogent Food Agric. Recio Published 30 May Medicine, Chemistry Planta medica Ellagic acid is a common metabolite present in many medicinal plants and vegetables.

It is present either in free form or as part of more complex molecules ellagitannins , which can be metabolized to liberate ellagic acid and several of its metabolites, including urolithins. While ellagic acid's antioxidant properties are doubtless responsible for many of its pharmacological activities, other mechanisms have also been implicated in its various effects, including its ability to reduce the… Expand.

View on PubMed. Save to Library Save. Create Alert Alert. Share This Paper. Methods Citations. Results Citations. Topics from this paper. Citation Type. Has PDF. Publication Type. More Filters. A Review. Ellagic acid a multi-target bioactive compound for drug discovery in CNS? Buy Powder. Related site Buy ellagic acid Beta Glucan. Ellagic acid tablets.

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